樱桃视频影院在线播放,免费中国帅气体育生GARY,野花韩国视频大全免费,欧美激情一区二区三区在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CCL-25WISH 細(xì)胞,人羊膜細(xì)胞

WISH 細(xì)胞,人羊膜細(xì)胞

簡要描述:WISH 細(xì)胞,人羊膜細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CCL-25
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1473

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

WISH 細(xì)胞,人羊膜細(xì)胞

ATCC® Number:CCL-25™    Price:$323.00
Additional information about this cell line

Designations:WISH

Depositors:L Hayflick

Biosafety Level:2

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: HeLa contaminant

Cellular Products:keratin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Virus Susceptibility:poliovirus 1, 2; adenovirus 3; vesicular stomatitis (Indiana)

Reverse Transcript:negative

DNA Profile (STR):Amelogenin: X

CSF1PO: 9,10

D13S317: 13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18



Isoenzymes:G6PD, A

HeLa Markers:Y

WISH 細(xì)胞,人羊膜細(xì)胞



Comments:This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

The cells are positive for keratin by immunoperoxidase staining.

NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination



Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C


Subculturing:Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.



Preservation:culture medium 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous c*tion. Exp. Cell Res. 23: 14-20, 1961. PubMed:13712490
26034: . . Am. J. Dis. Child. 103: 460, 1962.





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
中方县| 德阳市| 大宁县| 集贤县| 台北县| 宣威市| 厦门市| 阳东县| 裕民县| 五家渠市| 西宁市| 宜昌市| 徐汇区| 大同市| 额敏县| 峨边| 晋江市| 曲麻莱县| 曲水县| 灌阳县| 蒙自县| 惠安县| 曲靖市| 温宿县| 娱乐| 德兴市| 黎川县| 土默特左旗| 灵台县| 新龙县| 淮南市| 正定县| 洞口县| 霍州市| 深圳市| 晋江市| 都江堰市| 建始县| 湟中县| 和田市| 延川县|